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. 2017 Feb 9;7:42047. doi: 10.1038/srep42047

Figure 3. Optogenetic induction of site-specific methylation in DRG cells using DNMT3A-CRY2-EGFP.

Figure 3

(a) Pyrosequenced region at the TALE target sites of the Ascl1 promoter. The first CpG site is 41 bp upstream of the TALE binding region. The sequenced region is 166 bp from the transcription start site (TSS) of Ascl1 (b) DNA methylation level of DNMT3A-CRY2-EGFP and TALE co-transfected DRG cells in the sequenced region illustrated in (a). (c) Pyrosequenced region at the non-TALE targeting site. This sequenced region is 1.2 kb upstream of TALE target sites, and contains 3 CpG sites. Methylation in non-TALE target region was accessed in (d) DNMT3A-CRY2-EGFP and TALE co-transfected DRG cells. The extent of changes in methylation was compared between Non-transfected, transfected with no light, and transfection with light groups. Student’s paired t-test with two tails was used for statistical analysis. Data are presented as the mean ± SEM. N = 5 for each group. *p < 0.05; **p < 0.01.