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. 2017 Feb 9;7:42047. doi: 10.1038/srep42047

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Pyrosequenced region in the TALE target sites (b) DNA methylation level of TET1_-CRY2-EGFP and TALE co-transfected STR cells in the sequenced region illustrated in (a). (c) Pyrosequenced region in non-TALE targeting site. Methylation in non-TALE target region was accessed in (d) TET1_-CRY2-EGFP and TALE co-transfected STR cells. The extent of changes in methylation was compared between No-transfection, transfection no light, and transfection with light. Student’s paired t-test with two tails was used for statistical analysis. Data presented as mean ± SEM. N = 5 for each group. ^#P < 0.1; *P < 0.05; **p < 0.01.