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. 2017 Feb 9;7:42304. doi: 10.1038/srep42304

Figure 1. ASIC-knockout EAE mice showed pain comparable to the wild type despite different contribution to severity.

Figure 1

(a) EAE was induced in Asic1a−/− (N = 11), Asic2−/− (N = 13), Asic3−/− (N = 33) and littermate control Asic1a+/+ (N = 16), Asic2+/+ (N = 10), Asic3+/+ (N = 34) mice. Disease severity was monitored by daily clinical scoring for 30 days. (b) Mechanical sensitivity was assessed by von Frey test before EAE induction as baseline, 30 to 39 days post-induction and 40 to 49 days post-induction. (c) Regression line of peak score and cumulative score verses pain levels at 30 to 39 days and 40 to 49 days post-induction of EAE in wild-type, Asic1a−/−, Asic2−/− and Asic3−/− mice. Statistics were analyzed by two-way ANOVA. Genotype effect: Asic1a−/−: F(1,21) = 5.421, P = 0.03; Asic2−/−: F(1,20) = 1.778, P = 0.1974; Asic3−/−: F(1,65) = 0.4818, P = 0.4901; Time effect: Asic1a−/−: F(30,630) = 30.54, P < 0.001; Asic2−/−: F(30,600) = 42.30, P < 0.001; Asic3−/−: F(30,1905) = 120, P < 0.001. Interaction: no significance.