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. 2016 Nov 26;38(1):55–63. doi: 10.1002/humu.23123

Figure 4.

Figure 4

Effect of mutations on induction of PLCγ pathway. A: HeLa cells were transfected with wild‐type and mutant TRKA constructs and loaded with rhodamine‐3 and response to NGF was monitored. Ionomycin was used as the positive control. Only cells that did not spontaneously fluoresce and had an ionomycin response were used for analysis. WT, wild‐type. B: No significant increase in calcium levels was observed after NGF stimulation in p.G517E transfected cells. C: There was an increase in fluorescence in p.C752S transfected cells which peaked at t = 208. D: NGF stimulation on cells transfected with p.C763S caused an increase in fluorescence but the maximum value was reduced.