Figure 2. T-cell specific deletion of PARP-2 in a PARP-1-deficient background impairs T-cell homeostasis.

(A) Representative dot-plots of CD4, CD8, TCRβ, and CD24 expression in thymocytes from 8 to 10-week-old mice of the indicated genotypes. Percentage of cells in the individual subpopulations is indicated in each quadrant. (B) Graph showing the absolute number of thymocytes in each population. (C) Representative dot-plots of TCRβ, B220, CD4, CD8, CD44 and CD62L expression in splenocytes of the indicated genotypes. Percentage of cells in the individual subpopulations is indicated in each quadrant. (D) Graph showing the absolute number of splenocytes in each population. Naïve, CD62L⁺ CD44^lo; central memory, CD62L⁺ CD44^hi; effector memory, CD62L⁻CD44^hi. (E) Naïve/memory T-cell ratio in spleen. (F) Scheme describing the strategy for the generation of mixed bone marrow chimeras. Reconstituted cells were analyzed 10 weeks after transplantation. (G) Graph showing the frequency of CD45.2⁺ -expressing cells in spleen populations. Values represent the mean ± SEM of at least 8 mice of each genotype. *P < 0.05; **P < 0.01; ***P < 0.001.