Figure 6.

Creatine administration enhances oligodendrocyte restoration after focal spinal cord demyelination. A, B, Quantification of immunostaining for Nkx2.2⁺Olig2⁺ OPCS per square millimeter (A) and CC1⁺Olig2⁺ oligodendrocytes per square millimeter (B) at 5, 10, and 20 dpl in mice treated with PBS or 25 ng of creatine (CR). n = 3 mice/condition; Student's t test. C, Representative immunostaining of mature oligodendrocytes double positive for Olig2 (magenta) and CC1 (green) in PBS and CR lesions at 5dpl. Data are represented as mean ± SEM. Scale bars, 100 μm. Brightness and contrast were adjusted for visualization. n = 3 mice/condition; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.