Fig. 3. (A) DIC and fluorescence images of HeLa cells incubated with (E)-3/(Z)-3 and LysoTracker. The 405/525 channel and 552/625 channel are used to observed (E)-3/(Z)-3 and Lysotracker, respectively. Scale bar = 10 μm. (B) Normalized fluorescence intensity of (E)-3/(Z)-3 and LysoTracker over 30 seconds of irradiation (100 laser pulses of 300 ms). The 444/495 channel and 594/632 channel are used to for (E)-3/(Z)-3 and LysoTracker, respectively. Each set of data were fitted to a one phase exponential curve. The rate constant and half-life are 0.22 s^–1 and 3.15 s for (E)-3/(Z)-3 and 0.07 s^–1 and 9.75 s for LysoTracker (C) cell viability experiments of HeLa cells incubated with (E)-3/(Z)-3 and LysoTracker over 24 and 48 hours at 37 °C in a humidified atmosphere with 5% CO₂.