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. 2017 Feb 9;7:42130. doi: 10.1038/srep42130

Figure 5. NFI binding is associated with active chromatin near key BFC marker genes.

Figure 5

(A) Lipid accumulation visualised using Oil red O (ORO) staining of scrambled control versus Nfia KD samples. (B) UCSC browser screen shots of NFI binding sites at the Pparg2 promoter and distal to the Ppara promoter including H3K27ac ChIP-seq coverage profiles associated with Pre- and Mature- brown adipocytes. (C) Overview of NFI bound sites at their genomic locations at Day 0 and Day 4. (D) NFIA PWM derived from HOCOMOCO and used for scanning NFI peaks. (E) NFIA motif localization at Day 0 and Day 4, in a 1 kb region around the summit of NFI peaks. (F) NFI enrichment at Day 0 and Day 4 around 7,979 NFI bound regions across Day 0 and Day 4. (G) H3K27ac ChIP-seq enrichment within a 10 kb region surrounding NFI peaks enriched at Day 0 (group 1, 1,685 regions), common to both Day 0 and Day 4 (group 2, 4,546 regions) and enriched at Day 4 (group 3, 1,748 regions).