Figure 1. Autoantibodies are detected in some MA-EBOV surviving mice.

Sera from naïve mice (0) and MA-EBOV infected mice 2 or 3 weeks after challenge were screened for the presence of autoantibodies against tissue lysate by ELISA (A) or WB (B). (A) For ELISA, sera were diluted 50 fold, cumulative results, from 2 separate experiments are depicted (n = 8 per group). Dotted lines indicate 3 times the mean OD value for naïve sera (B) For WB, the following tissue lysate were analyzed by WB; lane 1-blood, 2-spleen, 3-liver, 4-kidney, 5-lung, 6-joints, 7-eyes, 8-brain, 9-muscle 10-ear, 11-heart. Membranes were incubated with 500 fold diluted naïve or MA-EBOV survivor serum or secondary antibody alone (2ary) as control. Representative blots from 4 separate experiments.