Table 2. Summary of ITC data.

Cell	Kd (nM)	ΔH	ΔS	n value	n
		Kcal/mol	cal/mol/deg
NaV1.5
WT	99 ± 4	−10.5 ± 0.1	−3.6 ± 0.2	0.99 ± 0.02	4
D1790G	128 ± 36	−10.5 ± 0.6	−2.4 ± 1.1	0.25 ± 0.04	3
Y1795C	283 ± 8a	−7.43 ± 0.2	4.6 ± 0.5	0.92 ± 0.02	3
Y1795H	103 ± 4	−10.2 ± 0.2	−3.0 ± 0.6	0.76 ± 0.02	3
E1901Q	243 ± 9a	−9.0 ± 0.2	−0.5 ± 0.6	0.85 ± 0.00	3
Q1909R	32,600 ± 1,300a	−14.9 ± 3.9	−37 ± 10	0.04 ± 0.02	3
R1913H	2,980 ± 570a	−9.0 ± 0.3	−5.3 ± 1.2	0.73 ± 0.03	3
L1917K	75 ± 29	−9.8 ± 1.0	−3.0 ± 0.6	0.98 ± 0.08	3
IQ/AA	Not detected	-	-	-	3
NaV1.2
WT	36 ± 6	−4.54 ± 0.1	18.6 ± 0.6	1.02 ± 0.02	3
H1853R	172 ± 20a	−8.3 ± 0.2	2.6 ± 1.0	0.30 ± 0.05	3
R1918H	112 ± 20a	−7.5 ± 0.2	6.2 ± 0.9	0.90 ± 0.40	3
NaV1.6
WT	276 ± 63b	−17.5 ± 0.3	−29.5 ± 1.1	0.24 ± 0.01	3

IQ/AA, IQ1908-1909AA.

^aP < 0.01 compared with WT.

^bP < 0.01 compared with Na_V1.5 WT.