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. 2017 Feb 8;17:20. doi: 10.1186/s12935-017-0388-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Proliferation-related characteristics and karyotype of TJ-GBC2 cell line. a The growth curve of TJ-GBC2 and SGC996 assayed using a MTT method. TJ-GBC2 cell line has a less vigorous growth tendency compared to SGC996 in vitro. b Cell cycle of TJ-GBC2 cell line detected by FCM, about 25% of the cells were in the S-G2/M phase. c Karyotype analysis of TJ-GBC2 cell line at the passage 50 (oil-immersion lens, ×1000): the number of chromosomes ranged from 48 to 132, with a peak number between 90 and 105, 80% of which is hypertetraploid