Table 3.
Summary of microbial community analysis conducted during phase II
| Treatment × species | n | # readsa | MED richnessb | MED evenness (J’)c | Total community structured | Detailed analysis | Top 50% MED coverage |
|---|---|---|---|---|---|---|---|
| PB × P. lobata | 2 | 142,167 (14,141) | 129 (35) | 0.40 (0.13) | VGl, PB: R = 0.75 VGl, VG: R = 1 VG, PB: R = 0.5 p = 0.001 for all |
No; low reads | – |
| VGl × P. lobata | 3 | 401 (161) | 86 (22) | 0.70 (0.07)d | |||
| PG × P. lobata | 1 | 641 | 100 | 0.61 | – | ||
| VG × P. lobata | 3 | 2594 (713) | 74 (16) | 0.29 (0.08) | Tested above | ||
| PB × P. verrucosa | 1 | 13,153 | 161 | 0.53 | – | No; low reads; low biological replication | – |
| VGl × P. verrucosa | 1 | 603 | 117 | 0.78 | – | ||
| PG × P. verrucosa | 3 | 745 (691) | 105 (19)d | 0.77 (0.16) | No differences | ||
| VG × P. verrucosa | 3 | 152 (23) | 65 (11) | 0.85 (0.04) | |||
| PB × A. humilis | 3 | 22,105 (20,156) | 131 (30) | 0.31 (0.21) | No differences | Yes | 0.78 |
| VGl × A. humilis | 2 | 21,133 (27,005) | 60 (2)d | 0.36 (0.41) | 0.67 | ||
| PG × A. humilis | 2 | 42,241 (53,217) | 85 (2) | 0.40 (0.45) | 0.67 | ||
| VG × A. humilis | 3 | 15,536 (11,314) | 59 (5)d | 0.27 (0.21) | 0.7 | ||
| PB × O. faveolata | 4 | 72,587 (25,401) | 378 (71) | 0.60 (0.16) | No differences | Yes | 0.84 |
| VGl × O. faveolata | 4 | 16,355 (18,421) | 237 (90) | 0.56 (0.17) | 0.71 | ||
| PG × O. faveolata | 4 | 28,852 (17,895) | 392 (87) | 0.66 (0.12) | 0.77 | ||
| VG × O. faveolata | 4 | 18,630 (9437) | 361 (43) | 0.61 (0.10) | 0.68 | ||
| PB × M. cavernosa | 0 | n/a | n/a | n/a | n/a | No; not all treatments represented | – |
| VGl × M. cavernosa | 2 | 65,040 (3908) | 405 (22) | 0.63 (0.12) | No differences | ||
| PG × M. cavernosa | 3 | 51,741 (26,956) | 366 (40) | 0.57 (0.04) | |||
| VG x M. cavernosa | 2 | 52,055 (7028) | 355 (115) | 0.67 (0.14) | |||
| PB × O. annularis | 3 | 87,777 (11,390) | 485 (60) | 0.73 (0.09) | No differences | Yes | 0.87 |
| VGl × O. annularis | 3 | 32,681 (4358) | 357 (45) | 0.74 (0.08) | 0.77 | ||
| PG × O. annularis | 3 | 24,582 (4974) | 313 (130) | 0.75 (0.15) | 0.67 | ||
| VG × O. annularis | 3 | 34,152 (8088) | 382 (123) | 0.71 (0.16) | 0.77 | ||
| PB × D. strigosa | 1 | 65,392 | 268 | 0.27 | n/a | No; not all treatments represented; low biological replication | – |
| VGl x D. strigosa | 0 | n/a | n/a | n/a | |||
| PG × D. strigosa | 1 | 60,393 | 211 | 0.23 | |||
| VG × D. strigosa | 1 | 41,726 | 172 | 0.18 |
All values are presented as mean (standard deviation (S. D.)) when appropriate. Single values with no S.D. represent samples from treatments with no replicates, and these values were not included in statistical significance testing
n = number of samples included in microbial community analysis after quality-filtering sequences
aAverage number of reads obtained for that species × treatment grouping out of the total number of analyzed reads
bSpecies superscripts within a column indicate significantly different MED richness between treatments (p < 0.05, one-tailed t test or one-way ANOVA with the Holm-Sidak method post hoc test)
cMED evenness values (J’) for each treatment with different superscripts indicate significant differences in species evenness (p < 0.05, one-way ANOVA with Holm-Sidak method post hoc test)
dDifferences in community structure were first determined using one-way ANOSIM global tests within each coral species (p < 0.05 is significance threshold). If significant differences were found, pairwise tests were conducted between the different treatments. Species × treatment combinations with only one sample were excluded in this analysis