Fig. 5.

Effect of REAC treatment on SOD (panel a) and MDA (panel b) intracellular concentrations of stallion spermatozoa during 72 h. of storage at 4 °C. For each parameter, the mean ± S.E values determined at the different time points are shown. A total of 16 ejaculates collected from eight stallions of different breeds (1: Thoroughbred; 2, 3, 4, 6: Arabian; 5, 7, 8: Warmblood) were used. Asterisks indicate statistical differences between REAC treated and untreated controls (General Linear Model): * p > 0.05; ** p < 0.001. Different letters indicate a statistical difference among values recorded at different time points within the same experimental group (General Linear Model): p > 0.01. Upper case letters: REAC group; lower case letters: control group