Figure 1. Testis cells express genes involved in bile acid synthesis.

(a) Plasma and testicular bile acid pool compositions in wild-type (n = 5 per group). (b) mRNA expression of Cyp7a1, Cyp8b1, Cyp27a1, Cyp7b1 and Hsd3b7 in purified Leydig cells and tubular compartment of testis normalized to β-actin mRNA levels in wild-type mice (n = 6–10 per group). (c) Testicular mRNA accumulation of Cyp7a1, Cyp8b1, Cyp27a1, Cyp7b1 and Hsd3b7 normalized to β-actin mRNA levels in whole testes of C57BL/6J mice treated with busulfan (20 mg/kg, one injection IP) at T0, 4, or 8 weeks (n = 8 per group). Data are expressed as means ± standard error of the mean. *Denotes significant difference from the T0 time point; #denotes significant difference from the 4-week time point (P < 0.05)%. In all panels, data are expressed as means ± standard error of the mean. Statistical analysis: *P < 0.05. *Denotes