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. 2017 Feb 9;7:42182. doi: 10.1038/srep42182

Figure 4. BA-diet induces male hypofertility in Fxrα−/− males in a Tgr5 independent manner.

Figure 4

(a) Testicular mRNA accumulations of Tgr5, Tbx2 and Cx43 normalized to β-actin mRNA levels in wild-type and Fxrα−/− mice fed a control or BA-diet for 1 month (n = 6 per group). Control diet groups were arbitrarily fixed at 100%. (b) Blood-testis-barrier integrity, as measured by the stained testes for EZ-link biotinylated. Representative micrographs of mice fed 1 month with a control diet or BA-diet. The original magnification was 100×. Quantification of the number of tubules with infiltration per 100 seminiferous tubules after 1 month of a control or BA-diet (n = 5 to 7 per group). (c) Testicular mRNA accumulation of Plzf, Stra-8, Dmc-1, and Tpn1 normalized to β-actin mRNA levels in wild-type and Fxrα−/− mice fed a control or BA diet for 1 month (n = 6 per group). In all panels, control diet groups were arbitrarily fixed at 100%. In all panels, data are expressed as means ± standard error of the mean. Statistical analysis: *P < 0.05.