Skip to main content
. 2017 Feb 9;7:42182. doi: 10.1038/srep42182

Figure 5. Fxrα is a gatekeeper of BA homeostasis in liver and testis in the context of BA exposure.

Figure 5

(a) Testicular concentrations of BAs (CA, tCA, DCA), the intermediate BA metabolites DHCA and 3β, 7α, 12 α tri-OH-5-cholestenic acid, in testis of Wt and Fxrα−/− males fed a control or BA-diet for 2 weeks (n = 5 per group). (b) mRNA accumulations of Pxr and Car in tubular compartment or purified Leydig cells of C57Bl6 males. (c) Testicular mRNA accumulation of Pxr and Car normalized to β-actin mRNA levels in wild-type and Fxrα−/− mice fed a control or BA-diet for 1 month (n = 6–10 per group). (d) Testicular mRNA expression of Cyp2b10, Mrp4, Mrp3, Aacs, Cyp26b1, Elmo1, Cyp17a1, Scd1 and Shp normalized to β-actin mRNA levels in wild-type and Fxrα−/− adult mice fed a control or BA-diet for 2 or 4 weeks (n = 6–10 per group). In all panels, control diet groups were arbitrarily fixed at 100%. In all panels, data are expressed as means ± standard error of the mean. Statistical analysis: *P < 0.05.