Figure 2.

Double labeling for Parvalbumin and GAD65-67 in the dentate gyrus (DG) of 12-months-old wt and TgproNGF#3 mice. (A) Quantification of GAD+/Parv+ neurons of wt and TgproNGF#3 mice (3- and 12-month old), expressed as percentage. Cell counting was performed on the DG on both sides, on at least 4–6 sections per animal, males and females (n = 4 per each animal group) (T-test, **P < 0.005). (B) Confocal Micrographs of Parv/GAD65-67 double immunofluorescence labeling in the DG of wt and TgproNGF#3 mice. Reduction of both Parv and GAD markers was observed. Scale bar: 50 micron.