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. 2017 Feb 9;10:20. doi: 10.3389/fnmol.2017.00020

Figure 4.

Figure 4

(A,B) The perineuronal nets (PNN) are markedly reduced in the dentate gyrus of TgproNGF#3 mice. Confocal microscopy. (A) Quantification of Parvalbumin+ interneurons of wt and TgproNGF#3 mice (3-, 6-, and 12-month-old). % total Parv+ neurons − blue bar − and Parv+ neurons ensheathed by the PNN − red bar. The reduction in Parv+ neurons ensheathed by PNN is significant in 6- and 12-month TgproNGF#3 mice. Analysis was performed on at least 4–6 sections per animal, males and females (n = 4 per each animal group) (T-test, *P < 0.005). (B) Double immunofluorescence for the perineuronal net and for Parvalbumin in the DG of 12-months-old wt and TgproNGF#3 mice. Brain sections were labeled with anti-Parvalbumin antibody, with the lectin Wisteria Floribunda (an agglutinin commonly used to map PNN and ECM, since it binds the glycan component of PNN proteoglycans) and Dapi. Scale bar: 50 micron. (C) Double immunofluorescence for aggrecan (a component of the PNN surrounding Parv+ interneurons) and for Parvalbumin in the DG of 12-months old wt and TgproNGF#3 mice. Scale bar: 50 micron.