Fig. 3. Metabolic switch occurs in DKO hearts.
Compared to WT, qRT-PCR analysis of DKO hearts revealed significant down-regulation of FAO genes Pgc1α, and m-Cpt1/2 (A–C). Additionally, the regulator of glucose oxidation Pdk4 was also altered in DKO hearts (D). All gene expression data were normalized to 36b4. (E–F) Line diagram showed a switch from oleate to glucose oxidation in the DKO whole hearts mounted ex vivo on Langendorff apparatus. (n=5/group; *p<0.05; ***p<0.0001 when compared to WT mice; Mean± SEM.)