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. Author manuscript; available in PMC: 2017 Feb 17.
Published in final edited form as: Hum Gene Ther Methods. 2014;25(1):48–56. doi: 10.1089/hgtb.2013.090

Fig. 5. Effect of hCD34+ cells prestimulation on the efficiency of lentiviral fusion and transduction.

Fig. 5

hCD34+ cells cultured overnight in absence (Mock) or presence of a cocktail of cytokines (see the Material and Methods section), were incubated 2.5 hr with VSV-G-BLAM-LVs (108 ig/ml), precoated for 2 hr on plate-bound Retronectin (7 μg/cm2). The viral fusion and transduction efficiency were estimated by measuring the level of cleaved CCF2 substrate (A) and the level of NGFR expression (B), respectively. Data are obtained from five different cord blood donors in simplicate or duplicate. Bars indicate the mean value of the distributions (** p<0.01, bilateral paired Student’s t-test).