Figure 9.

a) Encapsulin NCs for targeting the surface markers of HCC cells using SP94-peptide (blue) with linker (yellow) genetically or chemically attached (green) to the exterior surface of the NC. AlDOX is chemically attached to Encap_loophis42C123 and delivered to the target cell. Reprinted with permission from ref. ²³⁶ Copyright 2014 the American Chemical Society. b) Schematic showing integration of a light-responsive moiety (spiropyran) with an encapsulin NC at its surface through amine-succinimide reaction (i), and UV light-activated isomerization of spiropyran moiety (blue) with no fluorescence to merocyanine moiety (red) with high fluorescent activity. This reaction is reversible via a visible light irradiation (ii) Reprinted with permission from ref. ²⁴¹ Copyright 2016, John Wiley & Sons, Inc.