Figure 3.

Comparison of Ir-reducing assay with other methods. (a) Scheme illustrating commercial Cu-reducing assay. (b) Optical signal (absorbance) of Cu^OX when incubated with various reductants. (c) Optical signal increase of Cu^OX relative to the concentration of individual reductants. (d) GSH sensitivity of Ir-reduction assay compared with other methods (data from Ir-reduction and Cu-reduction assays were experimentally measured while the best fit lines were from Cao et al. (1998).⁴⁹ Measurements in parts b–d were performed in triplicate (all error bars indicate standard deviation).