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. 2016 Dec 30;51(3):1820–1829. doi: 10.1021/acs.est.6b05387

Figure 2.

Figure 2

Cytochrome P450-dependent metabolism of prochiral PCB 51 and PCB 102 to mono- and dihydroxylated metabolites is cytochrome P450 isoform and species dependent (analyzed as the corresponding methylated derivatives). The relative levels of hydroxylated metabolites of (A1) PCB 51 and (A2) PCB 102 differ between incubations with microsomal preparations from rats pretreated with inducers of different P450 enzymes. Moreover, relative levels of hydroxylated metabolites of (B1) PCB 51 and (B2) PCB 102 vary between incubations with liver microsomes obtained from different species. Levels of all OH-PCB metabolites were determined after methylation with diazomethane and are expressed relative to the internal standard because no authentic standards of the metabolites were available. Data are presented as mean ± standard deviation (n = 3 incubations per microsomal preparation; n = 2 for OH-PCB 102 hamster microsomal preparation incubations). Abbreviations of inducers: BNF, β-naphthoflavone; CFA, clofibric acid; DEX, dexamethasone; INH, isoniazid; PB, phenobarbital. ND: below the relative detection limit.