Table 1. Photophysical parameters for GCaMP6m.
εmax M-1cm-1 | φ | σ2 GM | n | pKa | |||||
---|---|---|---|---|---|---|---|---|---|
(λabs, nm) | (λfluor, nm) | (λabs, nm) | |||||||
Neutral | Anionic | Neutral | Anionic | Neutral | Anionic | Neutral | Anionic | ||
Ca2+-free | 37,300 | 73,600 | 0.041 | 0.56 | 140 | 54 | 0.97 | 0.03 | 8.01,10.08 |
(403) | (514) | (514) | (516) | (790) | (930) | 8.68* | |||
Ca2+-saturated | 37,700 | 83,100 | 0.048 | 0.63 | --- | 50 | 0.49 | 0.51 | 7.10 |
(397) | (497) | (512) | (513) | (930) | 6.90* | ||||
0.61* |
Extinction coefficient (ε); quantum yield (φ); two-photon cross-section (σ2); relative concentration of chromophore (n), previously reported values for GCaMP6m are in italics.
*[1]