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| GCaMP Transitions | Relevant Charge Changing Mutations | Experimental ΔΔpKa* | Calculated ΔΔpKa |
|---|---|---|---|
| GCaMP3 to GCaMP5A | D380Y | 0.50 ± 0.03 | 0.79 |
| GCaMP3 to GCaMP5D | R303P | 0.05 ± 0.06 | 0.02 |
| GCaMP5D to GCaMP5G | D380Y | 0.70 ± 0.08 | 0.79 |
| GCaMP5G to GCaMP6m | K379S, T381R, R392G | -1.27 ± 0.06 | -1.33 |
*[14]
