Fig 7. Identified compounds are specific high-risk HPV replication inhibitors.

U2OS cells were transfected with various types of HPV minicircle genomes and grown in the presence of the indicated concentrations of the compounds for 5 days. Genomic DNA was extracted, HPV DNA was linearized with the appropriate enzyme for each HPV type and bacterially produced input DNA was digested with DpnI. Replication signals were quantitated by qPCR (for HPV types 5, 11 and 16; panels A, B and C) or from Southern blots using a phosphoimager (for HPV types 31, 33 and 45; panels D, E and F) and are expressed relative to DMSO, as described in the Materials and Methods section. Panel G: CIN612E cells were grown in the presence of indicated concentrations of compounds for 6 days and HPV31 replication signal was quantitated from Southern blots using a phosphoimager and are expressed relative to DMSO. Error bars represent standard deviations from two to three independent experiments.