Fig 1. Ccdc40^lnks/lnks embryos show delayed then randomized expression of Lefty2 and Lefty1.

Lefty2 (A-D) and Lefty1 (E-H) expression in wild type (A, B, E, F) and Ccdc40^lnks/lnks (C, D, G, H) embryos at the 3- to 4- (A, C, E, G) and 5- to 7- (B, D, F, H) somite stages. Lefty1 and Lefty2 is expressed in the L-LPM in wild type embryos at both stages and Lefty1 is also expressed in the midline. In Ccdc40^lnks/lnks embryos, expression of Lefty1 and Lefty2 is not detected in the LPM at the 3- to 4-somite stages and expression is randomized between left-sided and bilateral at 5- to 7-somite stages. All embryos with bilateral expression of Lefty1 and Lefty2 showed weaker expression in the LPM. Expression of Lefty1 is weak in the midline of mutants at the 3- to 4-somite stage and mutants with bilateral Lefty1 expression show reduced expression in the midline. (I.) Quantitation of the number and percentage of embryos with absent (grey), left-sided (blue) and bilateral (magenta) Lefty1 or Lefty2 expression in the LPM. Left (L) and Right sides are labeled in panel A.