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Sera (diluted 1:100) was measured by ELISA from 10 fish per group (5 fish per duplicate tank) at 7 weeks post-immunization (wpi) (a) and at 3 weeks post-challenge (wpc) (b). Asterisks (*) represent p values (* = P<0.05, ** = P< 0.01, *** = P< 0.001) when compared to saline control group.
