Table 1. Rationale for protein selection.
| PROTEIN ID | RATIONALE OF SELECTION | FUNCTION | LOCATION | CONSERVATION (NCBI’s incomplete chromosomes) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| F | L | C | InterPro server | PSORTb server | CELLO server | % lc | h/s | M % sim. | SD % | |
| ASA_3320 | x | x | extracellular enzyme activity | unknown | periplasmic | 100 | [4/4] | 98 | 1 | |
| ASA_0826 | x | x | virulent enzymatic activity | extracellular | extracellular | 50 | [2/4] | 99 | 1 | |
| ASA_3455 | x | x | x | extracellular enzyme activity | extracellular | extracellular | 50 | [2/4] | 99 | 1 |
| ASA_3883 | x | x | x | iron sideophore receptor | outer membrane | outer membrane | 100 | [4/4] | 98 | 2 |
| ASA_2321 | x | x | heme binding | periplasmic | periplasmic | 100 | [4/4] | 98 | 2 | |
| ASA_0744 | x | x | pathogenesis, membrane | cytoplasmic | periplasmic + cytoplasmic | 100 | [4/4] | 97 | 2 | |
| ASA_1342 | x | x | motility | extracellular | periplasmic | 75 | [3/4] | 97 | 3 | |
| ASA_2532 | x | x | motility | unknown | periplasmic | 100 | [4/4] | 96 | 5 | |
| ASA_4042 | x | - | periplasmic | periplasmic | 100 | [4/4] | 92 | 9 | ||
| ASA_P5G035 | x | x | adhesion, pilin assembly | outer membrane | extracellular + outer membrane | 50 | [2/4] | 97 | 1 | |
| ASA_1675 | x | x | x | virulent enzymatic activity | extracellular | extracellular | 50 | [2/4] | 98 | 1 |
| ASA_3328 | x | x | x | haemoglobin/transferrin/lactoferrin receptor | outer membrane | outer membrane | 100 | [4/4] | 98 | 3 |
| ASA_3723 | x | x | x | collagenase | extracellular | outer membrane + cytoplasmic | 100 | [4/4] | 99 | 2 |
| ASA_4105 | x | x | zinc metallopeptidase | outer membrane | outer membrane | 100 | [4/4] | 98 | 2 | |
Fourteen proteins of A. salmonicida subsp. salmonicida were selected as potential protective B-cell antigens. The column RATIONALE OF SELECTION shows which criteria were responsible for the selection: functionality (F), location (L), conservation (C). E.g. protein ASA_2532 was selected due to its predicted motility function domain by InterPro and its strong conservation. Besides being present in the primary strain A449 (complete chromosome), it was represented in all NCBI’s incomplete chromosomes: number of homologs/number of strains (h/s). The % library coverage (% lc) of protein ASA_2532 is 100% meaning that this protein is 100% conserved across all sub-strains. Mean of % similarities to homologs (M % sim.) is 96% meaning this protein has an average % identity of 96% to the homologs of the 4 sub-strains. SD is the standard deviation of % similarities to homologs.