Table 3. Experimental setup presenting the different groups, sample size, mortality and relative percentage of survival (RPS).
| Control Saline | Control Adjuvant | Alpha Ject® 3000 | VacA | VacB | VacC | |
|---|---|---|---|---|---|---|
| No. of immunized fish | 60 | 60 | 60 | 60 | 60 | 60 |
| No. of fish sampled for ELISA (7wpi) | 10 | 10 | 10 | 10 | 10 | 10 |
| No. of challenged fish | 50 | 50 | 50 | 50 | 49* | 48* |
| Mortality % | 56 | 48 | 8 | 30 | 20 | 17 |
| RPS | - | 14 | 86 | 46 | 64 | 70 |
| No. of fish sampled for ELISA (3wpc) | 10 | 10 | 10 | 10 | 10 | 10 |
* Fish, one and two, respectively, died after anaesthesia during the challenge infection.