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. 2017 Feb 9;12(2):e0172026. doi: 10.1371/journal.pone.0172026

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© 2017 Aras et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — In vitro-matured M-II stage oocytes in control (A-C), in vitro ACR-treated (D-F) and in vivo ACR-treated (25 mg/kg) groups (G-I). Representative images in D-F were taken from a 1000 μM ACR-treated group. In vitro ACR-treated M-II stage oocytes showed no disruption in the meiotic spindle (green signal) and chromosome organization (red signal) appeared to be intact. In vivo ACR-treatment caused a decrease in spindle microtubule mass and disruption in the chromosome alignment. Multiple microtubule-organizing centers (MTOCs) were detected (arrows). PB: polar body. Scale bar: 10 μm.