Fig 3. Representative images of hESC spheroids prepared from TRA1-60+ purified cells.

Spheroids containing uniform numbers of cells were prepared in Aggrewell plates using TRA1-60⁺ cells. (A) Aggrewell plate containing spheroids aggregated for 3 days (1.25X magnification). (B) Aggrewell plate containing spheroids aggregated for 3 days (4X magnification). (C) Aggrewell plate containing spheroids aggregated for 3 days (10 X magnification). (D) Spheroids imaged immediately after aggregation.