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. 2017 Feb 8;19(3):226–236. doi: 10.1016/j.neo.2016.10.007

Figure 6.

Figure 6

H19 overexpression induced resistance to the treatment with 1,25(OH)2D3 in vivo. A, 1,25(OH)2D3 significantly inhibited the proliferation of cells stably expressing empty vector. The inhibitory effect didn't take place in cells stably expressing H19. The weight of tumors of mice/H19 cells were significantly increased compared with tumors of mice/mock cells (*P < .05, vs Mock + PG). B, The tumor growth curves of DLD-1 cells with stable overexpression of vector or H19 and treatment with 1,25(OH)2D3 or PG showed the same results as cell proliferation assays in vitro. H19 overexpression abrogated the inhibitory effect of 1,25(OH)2D3 on the proliferation of DLD-1 cells. C, Total RNA of tumors of mice/mock cells treated with either 1,25(OH)2D3 or PG was collected and real-time PCR was performed to measure the expression level of H19. Treatment with 1,25(OH)2D3 significantly inhibited the expression level of H19 in vivo. D, Total protein of tumors of mice/mock and mice/H19 treated with PG was collected and western blotting was performed to investigate the expression of VDR. H19 overexpression significantly inhibited the expression of VDR in vivo (*P < .05, vs control). All experiments were performed in triplicate and repeated at least three times. Results were expressed as mean ± SEM (n = 3).