Table 2. List of 35 recombinant M. avium subsp. paratuberculosis antigens.
| Nr. | Antigen | Description | E value | Ident. | Accession | Myco |
|---|---|---|---|---|---|---|
| 1 | MAP0139c | transcriptional regulator, PadR family protein [Mycobacterium avium 104] | 8,00E-144 | 99% | ABK65696.1 | MAA104 |
| 2 | MAP0586c | transglycosylase SLT domain protein [Mycobacterium avium 104] | 0 | 99% | ABK65241.1 | MAA104 |
| 3 | MAP0907 | morphine 6-dehydrogenase [Mycobacterium avium 104] | 0 | 98% | ABK68334.1 | MAA104 |
| MAP0907 | oxidoreductase [Mycobacterium avium subsp. hominissuis TH135] | 1,00E-52 | 41% | BAN32966.1 | MAH TH135 | |
| 4 | MAP1438c | alpha/beta hydrolase fold domain protein [Mycobacterium avium 104] | 0 | 99% | ABK65668.1 | MAA104 |
| MAP1438c | alpha/beta hydrolase [Mycobacterium avium subsp. hominissuis TH135] | 2,00E-24 | 40% | BAN33440.1 | MAH TH135 | |
| 5 | MAP1562c | conserved hypothetical protein [Mycobacterium avium 104] | 1,00E-89 | 100% | ABK66813.1 | MAA104 |
| 6 | MAP1693c-FL | peptidyl-prolyl cis-trans isomerase, fkbp-type domain protein [Mycobacterium avium 104] | 1,00E-125 | 98% | ABK64725.1 | MAA104 |
| MAP1693c-FL | peptidyl-prolyl cis-trans isomerase domain-containing protein [Mycobacterium avium subsp. hominissuis TH135] | 4,00E-113 | 100% | BAN31629.1 | MAH TH135 | |
| 7 | MAP2411 | pyridoxamine 5-phosphate oxidase [Mycobacterium avium subsp. hominissuis 10–4249] | 9,00E-101 | 100% | ETB30907.1 | MAH |
| 8 | MAP2677c | glyoxalase family protein [Mycobacterium avium 104] | 8,00E-92 | 98% | ABK69112.1 | MAA104 |
| 9 | MAP2746 | hypothetical protein MAH_1788 [Mycobacterium avium subsp. hominissuis TH135] | 4,00E-119 | 98% | BAN30862.1 | MAH TH135 |
| MAP2746 | conserved hypothetical protein [Mycobacterium avium 104] | 2,00E-109 | 99% | ABK64828.1 | MAA104 | |
| 10 | MAP-CF036-Ag3 | integral membrane protein YrbE1A [Mycobacterium avium subsp. hominissuis TH135] | 3,4 | 20% | BAN33502.1 | MAH TH135 |
| MAP-CF036-Ag3 | conserved hypothetical protein [Mycobacterium avium 104] | 3,4 | 20% | ABK66937.1 | MAA104 | |
| 11 | MAP3385 | methyltransferase [Mycobacterium avium subsp. hominissuis TH135] | 8,00E-65 | 64% | BAN32924.1 | MAH TH135 |
| MAP3385 | hypothetical protein MAH_3962 [Mycobacterium avium subsp. hominissuis TH135] | 2,00E-64 | 44% | BAN33036.1 | MAH TH135 | |
| MAP3385 | methyltransferase, putative, family protein [Mycobacterium avium 104] | 5,00E-32 | 40% | ABK67089.1 | MAA104 | |
| 12 | MAP3486 | lactate 2-monooxygenase [Mycobacterium avium 104] | 0 | 99% | ABK65670.1 | MAA104 |
| MAP3486 | LldD2 protein [Mycobacterium avium subsp. hominissuis TH135] | 4,00E-35 | 32% | BAN31520.1 | MAH TH135 | |
| 13 | MAP3547c | hypothetical protein MAV3388_23345 [Mycobacterium avium subsp. hominissuis 3388] | 0 | 99% | KDO92449.1 | MAH |
| MAP3547c | conserved hypothetical protein [Mycobacterium avium 104] | 2,00E-17 | 26% | ABK65078.1 | MAA104 | |
| 14 | MAP3680c | Formate dehydrogenase [Mycobacterium avium subsp. hominissuis TH135] | 0 | 99% | BAN33415.1 | MAH TH135 |
| MAP3680c | formate dehydrogenase [Mycobacterium avium 104] | 0 | 99% | ABK68013.1 | MAA104 | |
| 15 | MAP3731c | O-antigen export system ATP-binding protein RfbB [Mycobacterium avium 104] | 0,13 | 21% | ABK65477.1 | MAA104 |
| MAP3731c | O-antigen export system ATP-binding protein RfbB [Mycobacterium avium subsp. hominissuis TH135] | 0,16 | 21% | BAN29326.1 | MAH TH135 | |
| 16 | MAP3804 | glycosyl hydrolases family protein 16 [Mycobacterium avium 104] | 0 | 100% | ABK67366.1 | MAA104 |
| MAP3804 | glycosyl hydrolases family protein 16 [Mycobacterium avium subsp. hominissuis TH135] | 7,00E-174 | 100% | BAN33331.1 | MAH TH135 | |
| 17 | MAP4096 | nitroreductase family protein [Mycobacterium avium 104] | 6,00E-115 | 99% | ABK64565.1 | MAA104 |
| MAP4096 | nitroreductase [Mycobacterium avium subsp. hominissuis TH135] | 2,00E-04 | 23% | BAN29848.1 | MAH TH135 | |
| 18 | MAP4308c | fructose-bisphosphate aldolase class-I [Mycobacterium avium 104] | 0 | 99% | ABK68800.1 | MAA104 |
| 19 | Ag5 | hypothetical protein MAPs_16490 [Mycobacterium avium subsp. paratuberculosis S397] | 2,00E-166 | 100% | EGO37081.1 | MAP |
| 20 | Ag6 | MULTISPECIES: ATP-dependent helicase [Mycobacterium avium complex (MAC) | 0,047 | 39% | WP_038536272.1 | MAC |
| 21 | MAP1637c | M. avium UbD family decarboxylase | 0,00E+00 | 99% | WP_016706304.1 | M. avium |
| 22 | MAP0388 | hypothetical protein MAP_0388 [Mycobacterium avium subsp. paratuberculosis K-10] | 0 | 99% | AAS02705.1 | MAP |
| 23 | MAP3743-Ag9 | hypothetical protein MAP_3743 [Mycobacterium avium subsp. paratuberculosis K-10] | 0 | 99% | AAS06293.1 | MAP |
| 24 | MAP3744m-Ag10 | thiazolinyl imide reductase [Mycobacterium avium subsp. paratuberculosis 10–4404] | 0 | 99% | ETB06990.1 | MAP |
| 24 | MAP3744m-Ag10 | thiazolinyl imide reductase [Mycobacterium avium] | 0 | 99% | WP_016705779.1 | M. avium |
| 25 | MAP0217 | antigen 85-C [Mycobacterium avium 104] | 7,00E-180 | 99% | ABK64781.1 | MAA104 |
| MAP0217 | antigen 85-C protein [Mycobacterium avium subsp. hominissuis TH135] | 3,00E-179 | 99% | BAN29303.1 | MAH TH135 | |
| 26 | MAP0353 | glycerol kinase [Mycobacterium avium 104] | 0 | 99% | ABK67587.1 | MAA104 |
| 27 | MAP1166 | RecName: Full = Triosephosphate isomerase; Short = TIM; Short = TPI; AltName: Full = Triose-phosphate isomerase | 0 | 99% | A0QHY3.1 | MAA104 |
| 28 | MAP1362 | arginine biosynthesis bifunctional protein ArgJ [Mycobacterium avium 104] | 9,00E-145 | 99% | ABK65024.1 | MAA104 |
| 29 | MAP1803 | dienelactone hydrolase [Mycobacterium avium subsp. hominissuis TH135] | 4,00E-166 | 99% | BAN31765.1 | MAH TH135 |
| MAP1803 | dienelactone hydrolase family protein [Mycobacterium avium 104] | 2,00E-143 | 99% | ABK69268.1 | MAA104 | |
| 30 | MAP2800 | enoyl-CoA hydratase [Mycobacterium avium 104] | 0 | 99% | ABK64508.1 | MAA104 |
| MAP2800 | enoyl-CoA hydratase [Mycobacterium avium subsp. hominissuis TH135] | 2,00E-24 | 30% | BAN30137.1 | MAH TH135 | |
| 31 | MAP2864c | dihydrodipicolinate synthase [Mycobacterium avium 104] | 0 | 99% | ABK66322.1 | MAA104 |
| 32 | MAP3413 | aldehyde dehydrogenase (NAD) family protein [Mycobacterium avium 104] | 0 | 99% | ABK69290.1 | MAA104 |
| MAP3413 | P-cumic aldehyde dehydrogenase [Mycobacterium avium subsp. hominissuis TH135] | 3,00E-41 | 27% | BAN31590.1 | MAH TH135 | |
| 33 | MAP3634 | ErfK/YbiS/YcfS/YnhG family protein [Mycobacterium avium 104]Lipoprotein lprQ | 0 | 100% | ABK64614.1 | MAA104 |
| MAP3634 | hypothetical protein MAH_1464 [Mycobacterium avium subsp. hominissuis TH135] | 6,00E-48 | 41% | BAN30538.1 | MAH TH135 | |
| 34 | MAP3668c | phosphotriesterase-like protein [Mycobacterium avium subsp. hominissuis TH135] | 0 | 99% | BAN33426.1 | MAH TH135 |
| MAP3668c | phosphotriesterase-like protein [Mycobacterium avium 104] | 0 | 99% | ABK64738.1 | MAA104 | |
| 35 | MAP2541c | Malate dehydrogenase | 0 | 99% | A0QCI6.1 | MAA104 |
Ags 1–18: Map antigens identified by proteomic and immunoproteomic analysis of Map culture filtrate [26]; Ags 19–24: Map antigens identified by in silico predictions [27]; Ags 25–35: Map antigens identified in Map cultures submitted to stress conditions: nutrient starvation, hypoxia or acidic pH (Roupie et al., manuscript in preparation). Proteins actually upregulated in stress conditions are highlighted in grey. Putative function and results of BlastP comparison (E value and % protein identity) with Mah104 and Mah TH135 genome.