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. Author manuscript; available in PMC: 2017 Feb 9.
Published in final edited form as: Cell Signal. 2010 Jun 4;22(10):1502–1512. doi: 10.1016/j.cellsig.2010.05.019

Fig. 4.

Fig. 4

Expression of recombinant MK3 splice variants. (A) Aliquots of purified GST-MK3.1 and GST-MK3.2 were resolved by 10–20% acrylamide-gradient SDS-PAGE then stained with Coomassie blue. (B) Purified GST-MK3.1 and GST-MK3.2 were separated by 10–20% acrylamide-gradient SDS-PAGE, transferred to nitrocellulose and probed with antisera raised against GST (left) or MK3 (right). Numbers at the left indicate the positions of the molecular mass marker proteins (in kDa).