Figure 3.

The urinary mycobiome of asymptomatic control subjects. Clean-catch mid-stream urine samples were obtained from a panel of women without urinary complaints, as confirmed by validated urinary symptom questionnaires, then subjected to NGS using the ITS1 fungal ribosomal primer set. (A) The pie chart represents the overall prevalence of individual classes of fungi across the population as determined by taxonomic assignment using the THF database; (B) stacked bar-plot representation of the relative abundances at the class level of the urinary mycobiota of healthy subjects demonstrates significant interindividual variability as well as intraindividual diversity. NGS, next generation sequencing.