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. 2016 Sep 23;6(1):e00402. doi: 10.1002/mbo3.402

Figure 1.

Figure 1

Influence of FliA deletion or overexpression on transcription of bifA. (A) Swimming assay. Wild‐type KT2440, fliA mutant and fliA mutant complement cells were spotted onto swimming plates contained 0.4 mM IPTG. The photograph was taken 20 hr after inoculation at 28°C. (B) Growth (open symbols) and β‐galactosidase activity (filled symbols) of wild type (squares) and the fliA deletion mutant (triangles) harboring the bifA::lacZ fusion in pBBR‐bifApro‐lacZ. Results are averages and standard deviations from three experiments with duplicate samples. (C) Analysis of the influence of FliA overexpression on the transcription level of bifA by qRT‐PCR. Total RNA was extracted 3 hr after induction with 0.4 mM IPTG. Results are averages and standard deviations from three experiments with duplicate samples. **Statistically significant difference between FliA overexpression strain and the control strain (p < .01). (D) Heterologous expression of FliA in reporter Escherichia coli strain. Results are averages and standard deviations from three experiments with duplicate samples. **Statistically significant difference between β‐Galactosidase activities of pE and pC (p < .01)