Table 2.
Strains and plasmids used in this study
| Plasmids or Strains | Description | Source |
|---|---|---|
| Plasmids | ||
| pKSU | pKSV7 carrying the upp gene from B. subtilis 168, used for counterselection | Zhang et al. (2014) |
| pKSU‐Δsrf | pKSU carrying a mutant copy of the srf cluster | This study |
| pKSU‐Δitu | pKSU carrying a mutant copy of the itu cluster | This study |
| pKSU‐Δfen | pKSU carrying a mutant copy of the fen cluster | This study |
| Strains | ||
| B. amyloliquefaciens | ||
| LL3 | Glutamic acid‐independent poly‐γ‐glutamic acid (γ‐PGA)‐producing strain | Geng et al. (2011) |
| LL3Δupp | LL3 carrying an in‐frame deletion in the upp gene | Zhang et al. (2014) |
| LL3ΔpgsBCA | LL3 Δupp deleted for pgsBCA | Unpublished |
| LL3Δbae | LL3 Δupp deleted for its partial bae cluster | This study |
| LL3Δsrf | LL3 Δupp deleted for the srf cluster | This study |
| LL3Δitu | LL3 Δupp deleted for the itu cluster | This study |
| LL3Δfen | LL3 Δupp deleted for the fen cluster | This study |
| LL‐IS | LL3ΔuppΔitu deleted for the srf cluster | This study |
| LL‐ISF | LL‐IS deleted for the fen cluster | This study |
| LL‐ ISFB | LL‐ISF deleted for the bae cluster | This study |
| E. coli strains | ||
| DH5α | supE44 ΔlacU169(_80 lacZΔM15) recA1 endA1 hsdR17(rK− mK+) thi‐1gyrA relA1 F− Δ(lacZYA‐argF) | TransGen |
| GM2163 | F− dam‐13::Tn9 (Camr) dcm‐6 hsdR2 (rk −mk +) leuB6 hisG4 thi‐1 araC14 lacY1 galK2 galT22 xylA5 mtl‐1 rpsL136 (Strr) fhuA31 tsx‐78 glnV44 mcrA mcrB1 | Fermentas |