Table 3.
Oligonucleotide primers used in this study
| Primer names | Sequence (5′–3′)a |
|---|---|
| BaeUP‐F | CGGTCTAGAAAACTACATGTCATCTGTCATTAACG |
| BaeUP‐R | CATCGAGAAGTTCTTAAAAGATCCGGGCAGAC |
| BaeDN‐F | CCCGGATCTTTTAAGAACTTCTCGATGCCTAC |
| BaeDN‐R | TGAGTCGACGTGACGGCTTCTCTTTCAG |
| BaeOUT‐F | ATGATACCGCTCCATGTCAGCTCACTTG |
| BaeOUT‐R | CGCCGTGCTTCGTTCATCTAATTCG |
| SrfUP‐F | GCCGTCGACATGGGAATAACTTTTTATCC |
| SrfUP‐R | GGCATCGATATTGCTCCAGAGATACTGTAAAC |
| SrfDN‐F | CAGTATCTCTGGAGCAATATCGATGCCGATCG |
| SrfDN‐R | CGCGGATCCATCTTTAACCATTAAAGGAAAAG |
| SrfOUT‐F | GGAGGCTGTTTCTAAGGAAGAATTGAC |
| SrfOUT‐R | GACGTTTTATTTTGCCGGTCTGTTG |
| FenUP‐F | TGTGGATCCCTATCTTGCCCTCTGTCTTC |
| FenUP‐R | AGAAATATCCTTACGCAAACGGCAAAGTGGACC |
| FenDN‐F | TTTGCCGTTTGCGTAAGGATATTTCTGGTGCCG |
| FenDN‐R | GCAGTCGACTTGAAGAATACTGTTTATGCTT |
| FenOUT‐F | AATGGGTCAGCCGGTAGCTGGCAAG |
| FenOUT‐R | TGCGTCAAATTCAGGGGAAACATCG |
| ItuUP‐F | CGAGGATCCAAATTGAGGCAATAGGAATAG |
| ItuUP‐R | TAACAGTCAGTGTGTTGGGATCGTTTGCGGGAGAC |
| ItuDN‐F | GCAAACGATCCCAACACACTGACTGTTAAAATAGC |
| ItuDN‐R | CGAGTCGACTGGGGGCTTCACAATGATTTATGT |
| ItuOUT‐F | CGGTCATGTAGCCGATCTCACCTGG |
| ItuOUT‐R | ATTGAAATCTTCCGAATGGTGCTTG |
| qRpsU‐F | GTCGTTAGAAAAAACGAATCGCTTG |
| qRpsU‐R | TTGCGTTTTCTAGCAGCTTCTGACT |
| qPgsB‐F | TAGCCTGTGCTGCCGTACTAATCAT |
| qPgsB‐R | GTTTCCGTTTGATCGGTTTCTCCT |
a
Restriction sites used for the cloning of PCR amplicons are indicated in bold