Figure 5.

Analysis of the expression of arnC and arnD during growth on rich and starvation medium. (a) Samples of S. acidocaldarius were grown in rich medium, centrifuged and then transferred to either rich or starvation conditions and mRNA levels of arnC and arnD and were determined by qRT‐PCR at different time‐points. The increase in expression in the starvation medium compared to the rich medium is shown. Bars show the mean value of three independent biological replicates with each four technical replicates. (b) Western blot of the levels of ArnC‐HA and ArnD‐HA in the cytoplasm (C) and the membrane (M) after 4 hr of growth under rich (+) and starvation (‐) conditions. The membranes were concentrated 3.3‐fold compared to the cytoplasmic fraction. Proteins were detected using an antibody directed against the HA‐tag. The figure shows a representative blot of three independent replicates