Figure 6.
Expression and protein levels of FlaB and FlaX in rich and starvation medium. S. acidodaldarius was grown to an OD 600 of 0.4 in rich medium and transferred to either rich or starvation medium and samples for total RNA extraction and Western blot were collected. (a) Relative gene expression levels of flaB after 0.5 hr, 1 hr, 1.5 hr, 2 hr and 4 hr growth in rich medium compared to t = 0 of the WT (white bars) and the ΔarnC (gray bars), ΔarnD (light gray, dashed bars), and ΔarnCΔarnD (dark gray, dashed bars) strains. (b) Western blots of FlaB in whole cells grown in rich medium. Proteins were detected using an antibody directed against FlaB. A representative blot of at least three independent replicates is shown. (c) Relative gene expression levels of flaB after 0.5 hr, 1 hr, 1.5 hr, 2 hr and 4 hr growth in starvation medium compared to t = 0 of the WT (white bars) and the ΔarnC (gray bars), ΔarnD (light gray, dashed bars), and ΔarnCΔarnD (dark gray, dashed bars) strains. (d) Western blots of FlaB in whole cells grown in starvation medium. Proteins were detected using an antibody directed against FlaB. A representative blot of at least three independent replicates is shown. (e) Relative gene expression levels of flaX after 4 hr of growth in rich medium (dark gray bars) and starvation medium (light gray bars) compared to t = 0 of the WT and the ΔarnC, ΔarnD, and ΔarnCΔarnD strains. (f). Western blots of FlaX in whole cells grown in starvation medium and rich medium for 4 hr. Proteins were detected using an antibody directed against FlaX. A representative blot of at least three independent replicates is shown. Bars in (a), (c), and (e) represent the mean values of three independent biological replicates with each four technical replicates. Values that are significantly different compared to the WT (Student's t‐test, p ≤ 0.05) are indicated by asterisks