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. 2017 Jan 31;18(5):1171–1186. doi: 10.1016/j.celrep.2016.12.093

Figure 2.

Figure 2

miR-182 Is Active and Enriched in RGC Axons

(A) Sensor construct design.

(B) Schematic representation of the experimental protocol.

(C–E) Illustrative images of RGC GCs (C), RGC soma (D), or PRs (E) following retinal electroporation of control-Sensor or miR-182-Sensor. Clear examples of dGFP/mCherry ratio decrease are shown in (C) and (E).

(F and G) Quantification of the dGFP/mCherry ratio at the RGC GCs, soma, or PRs.

Values are mean ± SEM. Mann-Whitney test (F) and two-way ANOVA followed by Tukey post hoc test (G), ∗p < 0.05, ∗∗∗∗p < 0.0001. ns, nonsignificant; CMV, cytomegalovirus promoter; CS, complementary sequence; dGFP, destabilized GFP; INL, inner nuclear layer; PRL, photoreceptor layer; RGCL, retinal ganglion cell layer. Scale bars, 20 μm (B, D, and E) and 5 μm (C). See also Figure S2.