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. 2017 Jan 31;18(5):1171–1186. doi: 10.1016/j.celrep.2016.12.093

Figure 7.

Figure 7

Slit2 Inhibits miR-182 Activity in RGC Axons without Decay

(A, D, and F) Schematic representation of the experimental paradigm. Stage 35/36 retinal explants were cultured for 24 hr, and then Slit2 or vehicle were bath applied for 10 min.

(B) Illustrative images of GCs from miR-182-Sensor-electroporated RGCs grown in culture. A clear example of dGFP/mCherry ratio increase is shown in (B).

(C and E) Quantification of the dGFP/mCherry fluorescent ratio at the GC.

(G) Illustrative images of explants and axons before and after LCM.

(H) Illustrative gel of RT-PCR reaction for β-actin (β-act), MAP2, and histone H4 (H4) mRNA from cultured axons collected from stage 37/38 by LCM. In MAP2, H4, and β-act negative controls, PCR template was omitted.

(I) Quantification of miR-182 by the ΔΔCt method in LCM axons.

Values are mean ± SEM (C, E, and I). Mann-Whitney test, ∗p < 0.05. ns, nonsignificant; LCM, laser capture microdissection; RT−, RT no template negative control. Scale bars, 5 μm (B) and 200 μm (G). See also Figure S7.