Figure 2.
Probability of Syntaxin1a Molecule/LDCV Functional Overlap Is Low
(A) Molecule and vesicle positions derived from real data, with molecules represented in blue and vesicles in red.
(B) Molecular densities plotted for a 5 μm2 region of plasma membrane, illustrating the probability of molecules being present, represented in 2D (left) and 3D (right) plots.
(C) A method to measure overlap is shown graphically, using synthetic data, with molecules in red, vesicles in blue, and the result of combining the two in green, where the probability of a molecule localization is subtracted from the probability of a vesicle position (described in the Supplemental Experimental Procedures).
(D) Vesicles are predominantly in molecular gaps and overlap probability with SNARE/SM depots is low.
See also Figures S2 and S4.