Table 2.

Primers used in this study.

Primers	Oligonucleotide (5′→3′)a
capB2-up-F- BamHI	CGggatccGCACGCTCGGTGTTGTAAAG
capB2-up-R- HindIII	CCCaagcttCTTCTTCGTGTATTCGTCAT
capB2-down-F- HindIII	CCCaagcttGCATATTATGGGACTGATGA
capB2-down-R- XhoI	CCGctcgagTTGATCTAAGTCCTGCATGA
UP-F-attB2	GGGGACCACTTTGTACAAGAAAGCTGGGTGCACGCTCGGTGTTGTAAAG
Dn-R-attB1	GGGGACAAGTTTGTACAAAAAAGCAGGCTTTGATCTAAGTCCTGCATGA
SW-cp-F	ATAGTTTAGTAAGCGACCTG
XW-cp-R	TACAGGAAATCCGCATAAAT
Cp-BD-F	ATGACGAATACACGAAGAAG
Cp-BD-R	TCATGATTCATCAGTCCCAT
PstI-sarA-F	AAAActgcagTAAACCAAATGCTAACCCA
BamHI-sarA-R	CGCggatccAGTGCCATTAGTGCAAAAC
PstI-cap-19F	AAAActgcagATTTTTGTTACTAGTTTGT
BamHI-cap-19R	CGggatccTCATGATTCATCAGTCCCA
cap5K-F	GTCAAAGATTATGTGATGCTACTGAG
cap5K-R	ACTTCGAATATAAACTTGAATCAATGTTATACAG
cap8I-F	GCCTTATGTTAGGTGATAAACC
cap8I-R	GGAAAAACACTATCATAGCAGG
gyrB-F	ACATTACAGCAGCGTATTAG
gyrB-R	CTCATAGTGATAGGAGTCTTCT
cap-XF	ACGAATACACGAAGAAGTA
cap-XR	CCTCTGAAGTGATTACAATG
sarA-qtF	GTTATCAATGGTCACTTATGC
sarA-qtR	CTTGTGGTTGTTTGTAGTTT

^aRestriction sites are indicated by lowercase letters; underlined sequences denote recombination sites.