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. 2017 Feb 10;8:82. doi: 10.3389/fimmu.2017.00082

Figure 4.

Figure 4

NF-κB phospho-p65 formation 1–4 h after 2 Gy irradiation of 3D skin model. Nuclear translocation 1–4 h postirradiation detected by immunofluorescence (A). Green—p-p65 stain. Western blot analysis 1 h postirradiation shows high levels of p-p38 in the irradiated samples (B). The addition of 1 µmol/l Bay 11-7085 is suppressing the p-p65 formation (A,B). The graphs (B) represent the relative expression of p-p65 and p-p38 normalized to GAPDH detected by western blotting 1 h postirradiation. Results are arithmetical mean from two independent experiments. Each condition in these experiments had two replicate samples; error bars—SEM.