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. 2017 Feb 10;8:82. doi: 10.3389/fimmu.2017.00082

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Copyright © 2017 Acheva, Schettino and Prise.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Bay 11-7085 on cyclooxygenase-2 (COX-2) and p-p65 expression 4 h postirradiation of 3D organotypic skin cultures. The 1 µmol/l treated samples show the effect of the inhibitor on the COX-2 and p-p65 protein levels (A). The last three samples on each graph are increasing concentrations of the inhibitor plus 2 Gy 225 kVp X-ray irradiation. The positive irradiated control is 2 Gy. Graphs (B,C) represent the relative protein expression normalized to GAPDH. Results are arithmetical mean from two independent experiments. Each condition in these experiments had two replicate samples; error bars—SEM.