Figure 3.
Inhibition of mumps virus (MuV) replication by interferon β (IFN-β). (A) A dose-dependent IFN-β inhibition of MuV replication. Testicular cells were seeded in 6-well plates at a density of 2 × 105 cells/well and infected with one multiplicity of infection (MOI) MuV in the presence of the indicated doses of IFN-β. After 48 h, total RNAs were extracted, and relative RNA levels of MuV-NP were determined using real-time qRT-PCR. (B) Protein levels of MuV-NP. Cells were infected with 1 MOI MuV in the absence and presence of 200 U/mL IFN-β. At 48 h after MuV infection, MuV-NP was determined using Western blot analysis. (C) MuV titers. Cells were treated as described in (B). MuV titers in culture media were analyzed using plaque forming assay. The limit of detection was 10. Images represent at least three independent experiments. Data are presented as the means ± SEM of three experiments (*p < 0.05, **p < 0.01).