Figure 4.

Anti-TNF-α Antibodies Inhibit the Expression of Behavioral Fever

Carp (n = 15 per tank) were housed in 4 MCTs. On day 0, fish were infected with wild-type CyHV-3 (WT) and then injected 3 days later with control (Ctrl) (left column, 2 upper graphs) or anti-TNF-α (right column, 2 upper graphs) antibodies. Distribution of fish in the MCT compartments was analyzed and expressed as a mean per day + SD. The Ω symbol illustrates fish that died from the infection. See also Movie S3 recorded 6 dpi for the first replicate. The two lower graphs represent the global analysis of the data from the two replicates as described in Figure 2B. The days at which the number of fish per compartment was statistically different between the control and anti-TNF-α groups are indicated according to the level of significance.