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. 2017 Feb 10;8:165. doi: 10.3389/fpls.2017.00165

Figure 2.

Figure 2

T-DNA region of pBI101 containing CcCKS expression cassette and molecular confirmation of CcCKS transgenic Arabidopsis plants. (A) Restriction map of T-DNA region of pBI101 containing CcCKS expression unit driven by CaMV35S promoter and nptII selection marker. (B) PCR analysis for the presence of CcCKS gene in the genomic DNA of transgenic Arabidopsis plants. Lane M: 1.0 kb DNA size standard marker, Lane P, Positive control; Lane WT, wild type; Lanes 1–3, PCR amplification of CcCKS from transgenic plants. (C) RT PCR analysis showing the expression of kanamycin and CcCKS genes in transgenic Arabidopsis. Lane WT, wild-type; Lane VT, vector control; Lanes 1, 2, and 3: different transgenic lines. (D) Southern blot analysis of transgenic Arabidopsis plants carrying CcCKS gene. Lane WT, wild type plant; Lanes 1, 2, and 3: transgenic plants of three different lines (CS1, CS2, and CS3).